Reduced, valuable compounds can be produced using metabolically engineered microbes. The production of such molecules often relies on redox-cofactor-dependent biocatalysis in microbial production hosts. The prerequisite for outstanding performance requires a basic understanding of the redox metabolism of the production host, a simple redox chassis, and a target molecule. In this thesis, these prerequisites are addressed using the obligate aerobe Pseudomonas taiwanensis VLB120.