New developments in cosmology and biology offer exciting opportunities to explore the fundamental structure of the Universe, its physical origin and the origins of life. Vacuum energy, neutrinos and gravitational waves are fundamental ingredients of the physical Universe. The science of genomics and DNA explores the deep structure of life with its origins in cold and extreme environments. New advances in biology and computational science are driving exciting cross-disciplinary collaboration. This second Austria-France-Italy (AFI) symposium (Origins of the Universe and of Life) focussed on this science bringing together leading experts to review the status of these fields and prospects for fundamental new discoveries.