This detailed book explores techniques used in research efforts to adopt and combine the best capabilities from natural and artificial vector systems to assemble improved delivery technologies aimed for specific applications. The collection discusses vectors other than the traditional viral and non-viral systems and explores ideas for blending the best features of bacteria, nanoparticles, peptides, and hybrid systems for the delivery of biomaterials into cells, as well as a chapter on the application of shotgun proteomics and mass spectrometry as a tool to analyze the proteomic profile changes in cells that result from these interventions. Written in the highly successful format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, serves as an ideal guide for researchers working toward harnessing the power of multiple vectors in gene and drug delivery. weiterlesen