Part of the "Advanced Biotechnology" series, this volume covers all important aspects of cell culture engineering as applied to the production of recombinant proteins for therapeutic use, from molecular biology to bioprocess engineering. With a focus on mammalian and animal cells, the book describes the challenges in cell line development and cell engineering, while providing an overview of synthetic and systems biology approaches applied to cell culture engineering. In addition, the authors elaborate the use of CHO cells as common cell line for protein production and discuss current perspectives in production processes, including cell culture media, batch, fed-batch and perfusion processes, as well as process analytical technology, quality by design and scale down models. weiterlesen