This detailed book collects a variety of methods from investigators at the forefront of chondrocyte biology and pathology research that they have tailored for their research projects in order to tackle the unique properties and challenges of the chondrocyte and those of its tissue matrix. Divided into three parts, the volume explores experimental models to study chondrocytes, assays, as well as methods to characterize the chondrocyte phenotype with high-throughput or discrete assays on cells maintained , just isolated from their tissue (), or cultured . Written in the highly successful series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, is an ideal guide for experts as well as newcomers in the cartilage field with the aim of sparking new research ideas in order to solve many of the pending mysteries regarding cartilage biology, disease mechanisms, and potential treatments.weiterlesen