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CRISPR-Cas Methods

Produktform: Buch / Einband - flex.(Paperback)

This volume details the fundamentals of the , and its protocols illustrate advances in techniques for efficient genome editing. Introductory chapters provide a wide horizon of and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template. Authoritative and cutting-edge, aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.weiterlesen

Dieser Artikel gehört zu den folgenden Serien

Sprache(n): Englisch

ISBN: 978-1-07-160618-6 / 978-1071606186 / 9781071606186

Verlag: Springer US

Erscheinungsdatum: 05.07.2020

Seiten: 278

Auflage: 1

Herausgegeben von M. Tofazzal Islam, Pankaj K. Bhowmik, Kutubuddin A. Molla

128,39 € inkl. MwSt.
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lieferbar - Lieferzeit 10-15 Werktage

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