This new edition explores lab protocols describing new techniques to study cytotoxic T-cells (CTLs), as well as chapters of a more general discursive nature, all with an emphasis on the use of systems biology in immunology. Beginning with phenotypical characterization of CTL populations, the volume continues with and cytotoxicity assays, methods to detect senescent T cells, and models to understand immune and bone cells cross-talk, microscopy and imaging, as well as “Omics” approaches and molecular methods, concluding with chapters on CTL involvement in transplantation and link microbiota-immunity. Written for the highly successful series, chapters feature the kind of detail and key implementation advice for best results in the lab. Authoritative and up-to-date, serves as an ideal guide for researchers working with these vital cells.weiterlesen