This extensive new edition presents protocols reflecting the great strides made in the study of induced pluripotent stem (iPS) cells. The collection explores new and improved methods for the generation, expansion, and maintenance of iPS cells from different tissue types, characterization of their differentiation pathways along different lineages, and their potential utility in tissue repair and regeneration. Written for the highly successful series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, aims to arm stem cell biologists, both novice and expert, with invaluable protocols that are currently being used in various laboratories around the world. Chapters 37 and 52 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.weiterlesen