This book serves as a practical, detailed handbook on the application and analysis of isotopic tracers currently used in experimental biology and clinically-relevant disciplines. It is designed to be an accessible and valuable guide for students, researchers with little or no background using these techniques, and teachers interested in the application of isotopic tracers in experimental and diagnostic research. Each chapter includes background, principles, detailed methodology including the required reagents and step-by-step experimental analysis, and study questions which reinforce core concepts. These techniques have been carefully chosen based on their significance in the analysis of cellular and molecular biochemistry, and human pathophysiology. weiterlesen