Although previously thought to be merely passive structural components, membrane lipids have recently been found to be actively involved in cellular transport and signal transduction processes. Clear protocols for the study of membrane lipid properties, cellular transport or signal transduction are presented in this manual. Following a short introduction to membrane lipids, techniques for the isolation and extraction of membrane fractions, the analysis of the lipid composition, lipid turnover, and the involvement in signal transduction as well as the preparation of liposomes are described.weiterlesen