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Rapid Cycle Real-Time PCR — Methods and Applications

Genetics and Oncology

Produktform: E-Buch Text Elektronisches Buch in proprietärem

Rapid-Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I often allows product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms (SNPs). The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and diagnostics in the fields of genetics and oncology. Molecular diagnostics has never been easier!weiterlesen

Elektronisches Format: PDF

Sprache(n): Englisch

ISBN: 978-3-642-59397-0 / 978-3642593970 / 9783642593970

Verlag: Springer Berlin

Erscheinungsdatum: 29.06.2013

Seiten: 205

Herausgegeben von W. Dietmaier, Carl Wittwer, N. Sivasubramanian, C. Wittwer

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