This collection provides methods and techniques employed when culturing, manipulating, quantifying, and functionally assessing retinal ganglion cells (RGCs), vital for better understanding various ocular diseases and for developing novel therapies. The book features exhaustive and detailed protocols for the study of RGCs at both the and level, including the culture of rodent and human cells, and immunohistochemical, morphological, and functional assessments of various models of RGC death. Written for the highly successful series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, serves as an ideal guide to these important cellular features of the central nervous system and the many traumatic and degenerative blinding diseases that affect them.weiterlesen