Sébastien Peter Spectro–Microscopy of Autofluorescent Biomolecules Dissertation der Mathematisch-Naturwissenschaftlichen Fakultät der Eberhard-Karls-Universität Tübingen. Band 9 der Schriftenreihe LIFE SCIENCES. 182 Seiten. Zahlreiche, zum Teil farbige Abbildungen. Hardcover. Format B5. Preis: 44,00 Euro. ISBN 978-3-944101-99-6. Rhombos-Verlag, Berlin 2014 Fluorescence microscopy has become an indispensable tool in life sciences. The information within a microscopic image can be largely extended by the simultaneous acquisition of spectral information from a microscopic volume because fluorophores act as sensors to their nano-environment. In this work, different classes of autofluorescent biomolecules are spectro–microscopically investigated. It is shown that the photophysical properties of GFP derivatives largely depend on the composition of the surrounding protein shell, allowing for a better understanding of these proteins. Furthermore, the spectro–microscopic investigation of photosystems in living plant cells enables the observation of adaptation processes of plants to external and internal growth conditions. The parallel implementation and establishment of novel spectro–microscopic modalities further extends the accessible parameter space, offering even deeper insights into the photophysics of autofluorescent biomolecules. Key words: Fluorescence, Spectroscopy, Microscopy, Green Fluorescent Protein, Spectral Imaging Dekan: Professor Dr. Wolfgang Rosenstiel Mathematisch-Naturwissenschaftliche Fakultät an der Eberhard-Karls-Universität Tübingen http://www.ti.uni-tuebingen.de/Wolfgang-Rosenstiel.191.0.html 1. Berichterstatter: Prof. Dr. Alfred J. Meixner Eberhard-Karls-Universität Tübingen, Institut für Physikalische und Theoretische Chemie http://www.uni-tuebingen.de/Meixner/ 2. Berichterstatter: Prof. Dr. Klaus Harter Zentrum für Molekularbiologie der Pflanzen (ZMBP) an der Eberhard-Karls-Universität Tübingen http://www.uni-tuebingen.de/de/5078 3. Berichterstatter: Prof. Dr. Thomas Huser Fakultät für Physik, Arbeitsgruppe Biomolekulare Photonik, Universität Bielefeld http://www.physik.uni-bielefeld.de/biopho/index.php/en/weiterlesen